Remote Magnetic Microengineering and Alignment of Spheroids into 3D Cellular Fibers

Developing in vitro models that recapitulate the vivo organization of living cells a 3D microenvironment is one current challenges field tissue engineering. In particular for anisotropic tissues where alignment precursor required them to create functional structures. Herein, new method proposed allows aligning direction uniform magnetic both individual (muscle, stromal, and stem cells) or spheroids thermoresponsive collagen hydrogel. an all-in-one approach, are generated at high throughput by engineering using microfabricated micromagnets used as building blocks structures different scales. The process optimized terms cell labeling, concentration, size. Anisotropic induced form fibers alignment, with respective roles field, mechanical stretching hydrogel co-culture aligned non-magnetic stromal cells, being investigated. Over days, fuse into tubular structures, oriented alignment. Moreover, case muscle model, multinucleated can be observed within fibers.